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1 | PEB (4x) | protein extraction buffer

Каталожный номер
AS08 300
Производитель
Количество
5 x 2 ml (4x stock) allows up to 75 isolations of plant material (using 500 µl 1x PEB for 100 mg fresh weight) or 190 isolations of algal material (using 200 µl 1x PEB for cell amounts corresponding to 4-10 µg total chlorophyll)
Условия хранения
Stable at RT for at least 1 month; short-term storage (6 month) at 4
Подтвержденное взаимодействие
PEB has been tested on a wide range of species and tissues from Higher plants, Mosses, Llichens, Algae, Diatoms, Dinoflagellates, Cyanobacteria. Extracts may be quantified using detergent (LDS) compatible methods, and have been shown to give highly reprod
Происхождение
PEB is an extraction buffer for disruption and solubilisation of total protein from plant tissue and algal cells. The use of the anionic detergent LDS together with the recommended procedure (combination of sonication and freeze/thaw cycles) has been show
Область применения
23 203,76 руб.

Описание

Buffer components (4x): contains ~ 40% v/v glycerol [HOCH2CH(OH)CH2OH], Tris-HCl [NH2C(CH2OH)3 · HCl] pH 8.5, LDS [CH3(CH2)11OSO3Li], EDTA [(HO2CCH2)2NCH2CH2N(CH2CO2H)2]It is recommended to include a protease inhibitor (not supplied with this buffer) from

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